Evaluation of Nootropic Activity of Hydroalcoholic Fruit Extract of Annona Reticulata Linn. In Scopolamine induced Cognitive Impairment in Mice

Description

Memory and cognitive decline are hallmark features of Alzheimer’s disease, and current synthetic cholinesterase inhibitors raise tolerability and toxicity concerns, prompting interest in plant-derived alternatives. This study evaluated the nootropic potential of the hydroalcoholic fruit extract of Annona reticulata in scopolamine-induced cognitive impairment. Thirty-six male Wistar mice were divided into six groups (n=6): normal control, piracetam (400 mg/kg), scopolamine (1 mg/kg, i.p.), and three extract doses (100, 200, 400 mg/kg, p.o.) with scopolamine. Spatial learning and memory were assessed using the Morris Water Maze, followed by hippocampal histopathology; data were analyzed by ANOVA (mean ± SEM). The extract produced significant, dose-dependent improvement in escape latency and target-quadrant occupancy, with the 400 mg/kg dose approaching piracetam-treated performance and preserving hippocampal architecture. These findings indicate that A. reticulata fruit extract possesses notable cognitive-enhancing and neuroprotective activity, warranting further mechanistic investigation.

Keywords: Annona reticulata, Nootropic activity, Cognitive impairment, Scopolamine, Morris water maze, Neuroprotection.

Authors

DOI: 10.5281/zenodo.20688364

Publication Date: 2026-06-14

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